Journal: Cell

Article Title: Peptidoglycan Production by an Insect-Bacterial Mosaic

doi: 10.1016/j.cell.2019.08.054

Figure Lengend Snippet: A Complete PG Biosynthesis Pathway Is Predicted by Genomics (A) Schematic representation of a single P. citri bacteriocyte (blue), where Moranella cells and their two lipid bilayers (green) reside inside of triple-membrane-bound Tremblaya cells (yellow). (B) Adapted from Typas et al., 2011 . A pictorial representation of the genes present and expressed on the genomes of P. citri (blue represents native eukaryotic genes, brown represents HGTs of Gammaproteobacteria origin, orange represents HGTs of Bacteroidetes origin, and yellow represents Alphaproteobacteria origin) and Moranella (green) that are involved in PG production. The locations of the small colored cell schematics labeled with gene names are based on the predicted location of the protein product of that gene. See also .

Article Snippet: A custom polyclonal antibody was generated (ProSci Incorporated) to a peptide sequence (FVKSLENDYQKTKE) from the P. citri murF gene (accession: AGR65718) that was predicted to be surface exposed.

Techniques: Membrane, Labeling

Journal: Cell

Article Title: Peptidoglycan Production by an Insect-Bacterial Mosaic

doi: 10.1016/j.cell.2019.08.054

Figure Lengend Snippet: Representative Setup for Rearing and Feeding Experiments Involving P. citri , Related to , , , and Mealybugs (yellow arrows) were allowed to feed and reproduce on sprouted potatoes. Where treatments were applied, potatoes (often focusing on the sprouts) were covered and/or injected with 1 mL of the given treatment (modified D- or L-ala, cefsulodin, or water). Mealybugs were carefully placed on the potato with a paintbrush and allowed to eat ad libitum . Treatments were repeated each day for one week.

Article Snippet: A custom polyclonal antibody was generated (ProSci Incorporated) to a peptide sequence (FVKSLENDYQKTKE) from the P. citri murF gene (accession: AGR65718) that was predicted to be surface exposed.

Techniques: Injection, Modification

Journal: Cell

Article Title: Peptidoglycan Production by an Insect-Bacterial Mosaic

doi: 10.1016/j.cell.2019.08.054

Figure Lengend Snippet: MurF, a PG-Related HGT of Alphaproteobacterial Origin, Is Localized to the Moranella Cytoplasm Representative confocal image of a sectioned bacteriome stained with an anti-MurF antibody (red). Insect nuclei are stained with Hoechst (blue). Signal is detected inside of the Moranella cells and insect tissue, but not Tremblaya. Scale bar, 10 μm.

Article Snippet: A custom polyclonal antibody was generated (ProSci Incorporated) to a peptide sequence (FVKSLENDYQKTKE) from the P. citri murF gene (accession: AGR65718) that was predicted to be surface exposed.

Techniques: Staining

Journal: Cell

Article Title: Peptidoglycan Production by an Insect-Bacterial Mosaic

doi: 10.1016/j.cell.2019.08.054

Figure Lengend Snippet:

Article Snippet: A custom polyclonal antibody was generated (ProSci Incorporated) to a peptide sequence (FVKSLENDYQKTKE) from the P. citri murF gene (accession: AGR65718) that was predicted to be surface exposed.

Techniques: Plasmid Preparation, Recombinant, Electron Microscopy, DNA Library Preparation, Amplification, Software

Journal: PLoS ONE

Article Title: Implication of β2-adrenergic receptor and miR-196a correlation in neurite outgrowth of LNCaP prostate cancer cells

doi: 10.1371/journal.pone.0253828

Figure Lengend Snippet: (A) qRT-PCR analysis of ADRB2 in stably transfected LNCaP cells, shADRB2-1, shADRB2-2 and shADRB2-3, and negative control cells, shCtrl. A representative experiment with triplicates is shown. (B) qRT-PCR analysis of miR-196a in stably transfected LNCaP cells, shADRB2-1, shADRB2-2 and shADRB2-3, and negative control cells, shCtrl. (C) qRT-PCR analysis of ADRB2 in shADRB2-2 cells transfected with a plasmid overexpressing ADRB2 (shADRB2-2 p-ADRB2) and negative control cells (shADRB2-2 p-Empty). (D) qRT-PCR analysis of miR-196a in shADRB2-2 cells transfected with a plasmid overexpressing ADRB2 (shADRB2-2 p-ADRB2) and negative control cells (shADRB2-2 p-Empty). (E) qRT-PCR analysis of miR-196a in LNCaP Ctrl cells treated with different β-blockers or vehicle for 48 hours. The experiments were performed three times and a representative experiment showing the mean ± standard deviation of three replicates is presented in the figure. miR-196a expression was normalized to the reference gene miR-16-5p. The ADRB2 mRNA levels were normalized to the reference gene ALAS1.

Article Snippet: Briefly, LNCaP shADRB2-2 cells were transfected for 48h using XtremeGene HP DNA transfection reagent (cat. no. 06366236001; Sigma, St. Louis, MO) under androgen-proficient conditions with a pcDNA3 plasmid containing the full length ADRB2 gene (p-ADRB2) (cat. no. 14697; Addgene, Watertown, MA).

Techniques: Quantitative RT-PCR, Stable Transfection, Transfection, Negative Control, Plasmid Preparation, Standard Deviation, Expressing